Structure of sea urchin sperm chromatin core particle.

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Structure of sea urchin sperm chromatin core particle.

Sea urchin sperm chromatin contains forms of H1, H2A, and H2B which differ from those present in adult tissues. We have delineated some effects of the variant H2A and H2B on chromatin by study of the structure of the core particle from Strongylocentrotus purpuratus sperm. The particle contains 145 base pairs of DNA and equal amounts of the four smaller histones. It sediments at 11 S and has a c...

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The Lubrol-dispersed guanylate cyclase from sea urchin sperm was purified and isolated essentially free of detergent by GTP affinity chromatography, DEAE-Sephadex chromatography, and gel filtration. After removal of the detergent, the enzyme remained in solution in the presence of 20% glycerol. The specific activity of the purified enzyme was about 12 pmol of guanosine 3’:5’-monophosphate (cycl...

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Alterations in chromatin structure during early sea urchin embryogenesis.

Sea urchin sperm before fertilization possess the longest nucleosome repeat length yet determined for any chromatin. By the time the fertilized egg gives rise to a blastula or gastrula embryo, the chromatin has a considerably shorter repeat length and, in addition, a sequence of different histone variants of H1, H2A, and H2B has appeared. We have investigated the relationship between these vari...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1980

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)70364-0